Single cell analysis in long-read sequencers will revolutionize the understanding of biology and human disease with a huge impact on diagnostics and treatments. But this application is not possible yet due to the lack of an accurate method for error-prone long-read sequencers.
We developed a robust method to overcome the errors and unlock the true potential of long- read sequencers. With synthetic DNA tags (barcodes) we carefully re-engineered from mathematical elements used in digital communications. We have validated our core technology sequencing 4000 barcodes simultaneously. This is the highest ever number for a long-read sequencer and best of all we achieved high quality results that fall in the range of Illumina sequencing.